產(chǎn)品介紹

Biocolor 硫酸氨基聚糖檢測(cè)試劑盒 
Biocolor Blyscan™ Glycosaminoglycan Assay
 
    采用常規(guī)生化方法，通過標(biāo)準(zhǔn)曲線和比色，可定量分析總硫酸化多糖（氨基聚糖和蛋白聚糖）的含量，亦可測(cè)定O端和N端硫酸氨基多糖的比值。主要采用的是Blyscan染料（Biocolor公司的產(chǎn)品），它可以特異性的與氨基聚糖和蛋白聚糖上的硫酸多糖組分結(jié)合。該方法不適于檢測(cè)已經(jīng)降解的硫酸化雙糖片段。
    該試劑盒可檢測(cè)廣泛的蛋白多糖，可檢測(cè)的氨基多糖包括硫酸軟骨素、硫酸角質(zhì)素、硫酸皮膚素、硫酸肝素，不適合檢測(cè)包含海藻酸的樣品，因?yàn)樗鼈兒刑侨┧帷Ｔ撛噭┖幸膊荒軐?duì)透明質(zhì)酸定量，但透明質(zhì)酸并不干擾對(duì)硫酸化氨基多糖的檢測(cè)。細(xì)胞培養(yǎng)基中20%的胎牛血清也不會(huì)干擾實(shí)驗(yàn)。該方法檢測(cè)靈敏度為5ug，所需時(shí)間為1小時(shí)。
 
檢測(cè)樣本：可來(lái)自軟骨組織、動(dòng)脈組織、肺組織、皮膚組織、腫瘤組織、細(xì)胞核組織提取物、細(xì)胞培養(yǎng)液、羊水和尿液
 
實(shí)驗(yàn)操作 
1. 繪制標(biāo)準(zhǔn)曲線
2. 待測(cè)樣品的處理：
取50ul待測(cè)樣品進(jìn)行實(shí)驗(yàn)。如果反應(yīng)物在656nm初的吸收峰大于1.5，則對(duì)樣品進(jìn)行稀釋，待測(cè)樣樣品濃度落入標(biāo)準(zhǔn)曲線中段時(shí)，準(zhǔn)確度較高。
3. 硫酸氨基聚糖—染料復(fù)合物的獲得
加1.0ml的Blyscan染料溶液到待測(cè)樣本中，充分混勻，待不溶物出現(xiàn)，離心，棄上清，則獲得紫色的硫酸氨基聚糖—染料復(fù)合物
4. 硫酸氨基聚糖—染料復(fù)合物中染料的釋放
加入1.0ml的染料釋放劑，充分混勻。溶解5min以上，使染料充分釋放
5. 測(cè)定
用分光光度計(jì)于波長(zhǎng)656nm處測(cè)定，或使用colorimeter紅色濾光片測(cè)定
 
產(chǎn)品訂購(gòu)

   Product Code: B1000     110 assays 
   Product Code: B3000     440 assays

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